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Cellular MicroRNAs Inhibit Replication of the H1N1 Influenza A Virus in Infected Cells

In plasmid pRB849, the Escherichia coli lacZ gene replaced the γ134.5 open reading frame (ORF) contained in the BstEII-StuI fragment ofBamHI S (Fig. Following electrophoretic separation, the gel was silver stained, dried, and autoradiographed as described above and elsewhere (14). We found that the seed sequence of miR-26a was partially complementary to each of the PRRSV subgenomic sequences. Researchers concluded that all CD133− cells were capable of tumorigenesis; however, there was a trend toward lower tumor formation rates for GFAP+ and NSE+ cells, suggesting that their tumorigenic ability may involve other molecular distinctions aside from CD133 expression [24]. In brief, the RT reaction was performed using a PrimeScriptTM First Strand cDNA Synthesis Kit (Takara, Dalian, China) with an AlphaTM Unit Block Assembly for DNA EngineH systems (Bio-Rad, USA) under the following reaction